pE-FLP
pE-FLPBBF10K_000016
source
The flippase gene was amplified from pCP20 (e.g. available from the Coli Genetic Stock Center)
Can be used to remove the integration module (including the antibiotic resistance cassette) from pOSIP integrants.
attr.
Drew Endy, Keith Shearwin
Usage
growth
shipping strain
{shipping_strain}
growth conditions
For propagation, please grow this plasmid in the original strain (E811) In that strain, the strong promoter pE will be repressed, thus avoiding possible toxicity and accumulation of unwanted mutations in the plasmid. Use 50ug/ml ampicillin (100ug/ml may inhibit growth)
antibiotic
Ampicillin, low (50ug/mL)
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
N/A
enzyme
N/A
overhangs
3' - No Value … No Value - 5'
sequencing
forward primer
GGAATAAGGGCGACACGGAAATGTTGAATA
reverse primer
CCGTTACATATCAAAGGGAAAACTGTCC
Construct
plasmid name
pE-FLP
plasmid size
4389
insert size
N/A
origin
No Value
copy number
Low Copy
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere