AB_Pp-pGAP
Pichia GAP Promoter (enhanced)BBF10K_000180
source
P. pastoris
Pichia GAP Promoter using enhanced (AB) Freegenes syntax
attr.
Scott Pownall & Isaac Larkin
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - GGAG … TACT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-AB_Pp-pGAP
plasmid size
2600
insert size
599
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value