AB_Pp-pAOX1
Pichia AOX1 Promoter (enhanced)BBF10K_000181
source
P. pastoris
Pichia AOX1 Promoter using enhanced (AB) Freegenes syntax
attr.
Scott Pownall & Isaac Larkin
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - GGAG … TACT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-AB_Pp-pAOX1
plasmid size
3062
insert size
1061
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value