Cre
S. cerevisiae cds Cre recombinaseBBF10K_000207
source
P1 bacteriophage
For Cre-LoxP recombination, Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage.
attr.
Scott Pownall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-Cre
plasmid size
3159
insert size
1158
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No ValueReferences
Available Elsewhere
No Value