4S-limonene-synthase
S. cerevisiae CDS 4S-limonene-synthaseBBF10K_000238
source
Mentha spicata
4S-limonene-synthase (EC 4.2.3.16) catalyzes the cyclization of geranyl diphosphate to (‚àí)-(4S)-limonene. Limonene is a relatively stable colorless liquid aliphatic hydrocarbon classified as a cyclic monoterpene, and is the is a major component of the aromatic scents such as in the oil of citrus fruit peels.
attr.
Scott Pownall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-4S-limonene-synthase
plasmid size
3921
insert size
1920
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value