limonene-3-hydroxylase
S. cerevisiae cds limonene-3-hydroxylaseBBF10K_000243
source
Mentha spicata
(‚àí)-Limonene-3-hydroxylase (L3OH), using O2 and NADPH, catalyzes the allylic hydroxylation of (‚àí)-limonene at the 3 position to (‚àí)-trans-isopiperitenol.
attr.
Scott Pownall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-limonene-3-hydroxylase
plasmid size
3618
insert size
1617
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value