MMSYN1_0536
Ribosome-recycling factorBBF10K_000255
source
Synthetic bacterium JCVI-Syn3.0
Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.
attr.
Anton Jackson-Smith and Bioe80 class
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-MMSYN1_0536
plasmid size
2606
insert size
549
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE