carveol dehydrogenase
S. cerevisiae cds carveol dehydrogenaseBBF10K_000256
source
Mentha piperita
(-)-isopiperitenol/(-)-carveol dehydrogenase is capable of utilizing (‚àí)-trans-isopiperitenol in peppermint and (‚àí)-trans-carveol in spearmint. Carveol is a natural unsaturated, monocyclic monoterpenoid alcohol that is a constituent of spearmint essential oil in the form of cis-(‚àí)-carveol.
attr.
Scott Pownall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-carveol-dehydrogenase
plasmid size
3618
insert size
1617
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value