R-limonene synthase
S. cerevisiae cds R-limonene synthaseBBF10K_000264
source
No Value
(R)-limonene synthase (EC 4.2.3.20) catalyzes the synthesis of (+)-(4R)-limonene from GPP. This enzyme occurs in Citrus, Carum (caraway) and Anethum (dill).
attr.
Scott Pownall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-R-limonene-synthase
plasmid size
3948
insert size
1947
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value