ScGal4-DNA-AD
S. cerevisiae cds-Gal4-DNA-Activation-DomainBBF10K_000327
source
S. cerevisiae
Yeast-2-hybrid bait fusion - Gal4-DNA-Activation-Domain
attr.
Scott Pownall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - TCCAtg … ggAATG - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-ScGal4-DNA-AD
plasmid size
2469
insert size
468
origin
ColE1 High Copy
copy number
500-701
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value