N1N2_SV40_NLS
N1N2 SV40 Nuclear Localization Signal (NLS)BBF10K_000335
source
SV40
SV40 Nuclear Localization Signal (NLS) for Yeast-2-Hybrid or other fusions, targets the protein to the yeast nucleus.
attr.
Scott Pownall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - CCATg … ggGTCA - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-N1N2_SV40_NLS
plasmid size
2411
insert size
410
origin
ColE1 High Copy
copy number
500-703
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value