MMSYN1_0795
ATP synthase subunit cBBF10K_000349
source
Synthetic bacterium JCVI-Syn3.0
Key component of the F(0) channel; it plays a direct role in translocation across the membrane. A homomeric c-ring of between 10-14 subunits forms the central stalk rotor element with the F(1) delta and epsilon subunits.
attr.
Anton Jackson-Smith and Bioe80 class
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-MMSYN1_0795
plasmid size
2363
insert size
306
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE