Pp_aMF_sec_tag
Pichia aMF Secretion TagBBF10K_000355
source
Pichia (Komagataella pastoris)
Pichia aMF Secretion tag
attr.
Scott Pownall & Isaac Larkin
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - TACT … tCCAT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-Pp_aMF_sec_tag
plasmid size
2411
insert size
410
origin
ColE1 High Copy
copy number
500-704
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value