AConL-start
assembly connector left startBBF10K_000424
source
synthetic
For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design
attr.
Scott Pownall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI/BbsI
overhangs
3' - ATGA … GGAG - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-AConL-start
plasmid size
2318
insert size
317
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value