PpHR5'-NTS
Pichia NTS 3'-Homology RegionBBF10K_000455
source
Pichia (Komagataella pastoris)
for stable genome integration adjacent to Pichia NTS locus.
attr.
Scott Pownall & Isaac Larkin
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - AAAA … AAGG - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-PpHR3'-NTS
plasmid size
2416
insert size
415
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value