PpHR3'-RGI2
Pichia RGI2 5'-Homology RegionBBF10K_000458
source
Pichia (Komagataella pastoris)
for stable genome integration adjacent to Pichia RGI2 locus.
attr.
Scott Pownall & Isaac Larkin
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - AGAC … CGAA - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-PpHR5'-RGI2
plasmid size
2654
insert size
653
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value