ScLEU2-marker
marker-cassette-LEU2BBF10K_000471
source
No Value
Yeast selection marker, complements LEU2 auxotrophy
attr.
Scott Pownall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - AAGG … ATGA - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-ScLEU2-marker
plasmid size
4041
insert size
2040
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value