OYC-bridge-AGAC-CGAA
OYC-bridge-AGAC-CGAABBF10K_000476
source
No Value
Bridge for AGAC/CGAA, See design: https://docs.google.com/spreadsheets/d/1hhiKwaTJyWajH1fEUxZ_79DP4TRtlCBLvO6EtcqtxeY/edit?usp=sharing
attr.
Scott Pownall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - AGAC … CGAA - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-OYC-bridge-AGAC-CGAA
plasmid size
2411
insert size
410
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value