OYC-eforRed-dropout
OYC-eforRed-dropout-cassetteBBF10K_000482
source
No Value
eforRed selection cassette for level 1 vector, clones into backbone and used for colony selection following Level 1 assembly
attr.
Scott Pownall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
No Value
promoter
No Value
inducer
No Value
cloning
method
Golden Gate Assembly
enzyme
BsaI
overhangs
3' - GCAA … AAAA - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-OYC-eforRed-cassette
plasmid size
3147
insert size
1146
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
No Value