pOSIP-KO (KanR, 186)
pOSIP-KO (KanR, 186)BBF10K_000492
source
No Value
Please note that there is a E59G mutation in Integrase 186. The mutation does not affect device function.
attr.
Drew Endy, Keith Shearwin
Usage
growth
shipping strain
{shipping_strain}
growth conditions
Contains ccdB, so must be grown in a strain resistant to this product (e.g. DB3.1). Growth a 30C to repress expression of the integrase.
antibiotic
Kanamycin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
Restriction Ligation
enzyme
Multiple Cloning Site
overhangs
3' - No Value … No Value - 5'
sequencing
forward primer
TAAACTGCCAGGAATTGGGGATC
reverse primer
GAAACGCAAAAAGGCCATCCGTC
Construct
plasmid name
pOSIP-KO (KanR, 186)
plasmid size
6298
insert size
N/A
origin
pUC
copy number
High Copy
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere