NOR gate-V2.0
NOR gate-V2.0BBF10K_000499
source
No Value
Two-input recombinase logic NOR gate. Produces GFP only when no recombinase has been expressed and acted on the DNA registers (binary output number: 1000). Consists of a promoter followed by two transcriptors (asymmetric transcriptional terminators flanked by inverted recombination sites) in permissive orientation placed in series. When one or both terminators are inverted, GFP is not expressed anymore. Operated by recombinases Bxb1 and TP901 (e.g. using the dual controller). Low copy ppSC101 ori (5-10 copies)- Ampicilin resistance.
attr.
Drew Endy
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
N/A
enzyme
N/A
overhangs
3' - No Value … No Value - 5'
sequencing
forward primer
No Value
reverse primer
No Value
Construct
plasmid name
NOR gate-V2.0
plasmid size
5030
insert size
No Value
origin
pSC101
copy number
5-10
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere