XOR gate-V2.0
XOR gate-V2.0BBF10K_000500
source
No Value
Two-input recombinase logic XOR gate. Produces GFP when only one of two recombinases have been expressed and acted on the DNA registers (binary output number: 0110). Consists of a promoter followed by one transcriptional terminator flanked by nested recombination sites in an inverted orientation. The action of either recombinase inverts the terminator and allows for the expression of downstream GFP. The action of the second recombinase put the terminator back in blocking orientation. Operated by recombinases Bxb1 and TP901 (e.g. using the dual controller). Low copy pSC101 ori (5-10 copies)- Ampicilin resistance.
attr.
Drew Endy
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
N/A
enzyme
N/A
overhangs
3' - No Value … No Value - 5'
sequencing
forward primer
No Value
reverse primer
No Value
Construct
plasmid name
XOR gate-V2.0
plasmid size
4935
insert size
No Value
origin
pSC101
copy number
5-10
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere