B7H7
HHLA2 HERV–H LTR-associating protein 2BBF10K_000515
source
Human
ligand for CD28H
attr.
Nina Horowitz
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Kanamycin
expression
strain
Human
promoter
SFFV
inducer
N/A
cloning
method
Gibson assembly into viral backbone
enzyme
BamHI
overhangs
3' - CAGACTGAGTCGCCCGGGGGgatccgccacc … ggatccGCCACCAACTTCAGCCTGCT - 5'
sequencing
forward primer
CAGACTGAGTCGCCCGGGGGgatccgccacc
reverse primer
gcaggctgaagttggtggcggatcc
Construct
plasmid name
pOpen-B7H7
plasmid size
5787
insert size
1399
origin
oriS
copy number
medium
Safety
BSL
2
other information
No ValueReferences
Available Elsewhere
FALSE