TGFb
transforming growth factor beta 1BBF10K_000518
source
Human
immunostimulatory or inhibitory molecule
attr.
Nina Horowitz
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Kanamycin
expression
strain
Human
promoter
SFFV
inducer
N/A
cloning
method
Gibson assembly into viral backbone
enzyme
BamHI
overhangs
3' - CAGACTGAGTCGCCCGGGGGgatccgccacc … ggatccGCCACCAACTTCAGCCTGCT - 5'
sequencing
forward primer
CAGACTGAGTCGCCCGGGGGgatccgccacc
reverse primer
gcaggctgaagttggtggcggatcc
Construct
plasmid name
pOpen-TGFb
plasmid size
5715
insert size
1327
origin
oriS
copy number
medium
Safety
BSL
2
other information
No Value
References
Available Elsewhere
FALSE