PDGF-DDtv1
platelet-derived growth factor D transcription variant 1BBF10K_000519
source
Human
ligand for NKp44
attr.
Nina Horowitz
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Kanamycin
expression
strain
Human
promoter
SFFV
inducer
N/A
cloning
method
Gibson assembly into viral backbone
enzyme
BamHI
overhangs
3' - CAGACTGAGTCGCCCGGGGGgatccgccacc … ggatccGCCACCAACTTCAGCCTGCT - 5'
sequencing
forward primer
CAGACTGAGTCGCCCGGGGGgatccgccacc
reverse primer
gcaggctgaagttggtggcggatcc
Construct
plasmid name
pOpen-PDGF-DDtv1
plasmid size
5655
insert size
1267
origin
oriS
copy number
medium
Safety
BSL
2
other information
No ValueReferences
Available Elsewhere
FALSE