StrepCAR-CD28z
anti-streptavidin chimeric antigen receptor, intracellular CD28zBBF10K_000524
source
Human
activating receptor on NK cells to bind to biotinylated antibodies
attr.
Katarzyna Urbanska, Evripidis Lanitis, Mathilde Poussin, Rachel Lynn, Brian P. Gavin, Sander Kelderman, Jason Yu, Nathalie Scholler, and Daniel J. Powell, Jr
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Kanamycin
expression
strain
Human
promoter
SFFV
inducer
N/A
cloning
method
Gibson assembly into viral backbone
enzyme
BamHI
overhangs
3' - CAGACTGAGTCGCCCGGGGGgatccgccacc … ggatccGCCACCAACTTCAGCCTGCT - 5'
sequencing
forward primer
CAGACTGAGTCGCCCGGGGGgatccgccacc
reverse primer
gcaggctgaagttggtggcggatcc
Construct
plasmid name
pOpen-StrepCAR-CD28z
plasmid size
5658
insert size
1270
origin
oriS
copy number
medium
Safety
BSL
2
other information
No Value
References
Available Elsewhere
FALSE