CD19CAR-CD28z

anti-CD19 chimeric antigen receptor, intracellular CD28z
BBF10K_000527

source

Human

activating receptor on NK cells to bind to CD19

attr.

James N. Kochenderfer, Wyndham H. Wilson, John E. Janik, Mark E. Dudley, Maryalice Stetler-Stevenson, Steven A. Feldman, Irina Maric, Mark Raffeld, Debbie-Ann N. Nathan, Brock J. Lanier, Richard A. Morgan, and Steven A. Rosenberg

Usage

growth

shipping strain

{shipping_strain}

growth conditions

37 C, shaking 300 rpm

antibiotic

Kanamycin

expression

strain

Human

promoter

SFFV

inducer

N/A

cloning

method

Gibson assembly into viral backbone

enzyme

BamHI

overhangs

3' - CAGACTGAGTCGCCCGGGGGgatccgccacc … ggatccGCCACCAACTTCAGCCTGCT - 5'

sequencing

forward primer

CAGACTGAGTCGCCCGGGGGgatccgccacc

reverse primer

gcaggctgaagttggtggcggatcc

Construct

plasmid name

pOpen-CD19CAR-CD28z

plasmid size

6570

insert size

2182

origin

oriS

copy number

medium

genbank file

Safety

BSL

other information

No Value

References

primary references

NCBI

N/A

UniProt

N/A

Available Elsewhere

FALSE

protocols and instructions

No Value

citations

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2993617/?report=reader

intellectual property

We are unaware of third-party property rights claims on uses of this item as of .