CD19CAR-CD28z

anti-CD19 chimeric antigen receptor, intracellular CD28z
BBF10K_000527
source
Human

activating receptor on NK cells to bind to CD19

attr.
James N. Kochenderfer, Wyndham H. Wilson, John E. Janik, Mark E. Dudley, Maryalice Stetler-Stevenson, Steven A. Feldman, Irina Maric, Mark Raffeld, Debbie-Ann N. Nathan, Brock J. Lanier, Richard A. Morgan, and Steven A. Rosenberg

Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Kanamycin

expression

strain
Human
promoter
SFFV
inducer
N/A

cloning

method
Gibson assembly into viral backbone
enzyme
BamHI
overhangs
3' - CAGACTGAGTCGCCCGGGGGgatccgccacc … ggatccGCCACCAACTTCAGCCTGCT - 5'

sequencing

forward primer
CAGACTGAGTCGCCCGGGGGgatccgccacc
reverse primer
gcaggctgaagttggtggcggatcc

Construct

plasmid name
pOpen-CD19CAR-CD28z
plasmid size
6570
insert size
2182
origin
oriS
copy number
medium

Safety

BSL
2

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

protocols and instructions

No Value

intellectual property

We are unaware of third-party property rights claims on uses of this item as of .