pOMRP4β
sdhB_hph_GlucBBF10K_000571
source
G. lucidum; E. coli
Agrobacterium tumefaciens T-DNA conferring resistance to carboxin and hygromycin B to G. lucidum.
attr.
Rolando Perez
Usage
growth
shipping strain
{shipping_strain}
growth conditions
Grow at 37 C with shaking at 300 rpm to recover from E. coli.
antibiotic
Kanamycin
expression
strain
Ganoderma lucidum
promoter
GPD
inducer
N/A
cloning
method
Gibson Assemly
enzyme
N/A
overhangs
3' - N/A … N/A - 5'
sequencing
forward primer
gcctgtatcgagtggtgattttgtg
reverse primer
AATCGCGAGCTCACTAATCACCC
Construct
plasmid name
pOMRP4β
plasmid size
12874
insert size
3171; 1026
origin
pBR322 (E. coli), pVS1-REP (Agrobacterium)
copy number
No Value
Safety
BSL
1
other information
No Value
References
Available Elsewhere
FALSE