pInducible
pOpen_v4BBF10K_000589
source
Synthetic
Golden-Gate-compatible cloning vector. Low-copy, includes an indicuble high-copy origin. For cloning difficult parts.
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
Kanamycin
expression
strain
Escherichia coli
promoter
LacOP induces high copy
inducer
IPTG
cloning
method
GoldenGate
enzyme
BbsI, AarI
overhangs
3' - varies … varies - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen_Inducible
plasmid size
6238
insert size
No Value
origin
oriS, ColE1
copy number
low; high
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE