yD4E
S. cerevisiae codon optimized Dengue serotype 4 Envelope glycoproteinBBF10K_000596
source
Dengue virus 4
Binds to host cell surface receptor and mediates fusion between viral and cellular membranes
attr.
Nguyen Ngoc Luong
Usage
growth
shipping strain
{shipping_strain}
growth conditions
43 C, shaking 300 rpm
antibiotic
Kanamycin
expression
strain
Saccharomyces cerevisiae 2805
promoter
Gal1/TDH3-1
inducer
Galactose/No inducer
cloning
method
Restriction digestion
enzyme
BamHI/SalI
overhangs
3' - GGATCC … GTCGAC - 5'
sequencing
forward primer
No Value
reverse primer
No Value
Construct
plasmid name
pOPEN00237
plasmid size
No Value
insert size
1485
origin
E. coli OriC
copy number
500-700
Safety
BSL
1
other information
N/A
References
Available Elsewhere
No Value