glmU
bifunctional N-acetylglucosamine-1-phosphateuridyltransferase/glucosamine-1-phosphateacetyltransferaseBBF10K_000759
source
Bacillus subtilis 168
forms a homotrimer; catalyzes the acetylation ofglucosamine-1-phosphate and uridylation ofN-acetylglucosamine-1-phosphate to produce UDP-GlcNAc;function in cell wall synthesis
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-glmU
plasmid size
3394
insert size
1371
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE