ispD
2-D-methyl-D-erythritol 4-phosphatecytidylyltransferaseBBF10K_000769
source
Bacillus subtilis 168
4-diphosphocytidyl-2C-methyl-D-erythritol synthase;MEP cytidylyltransferase; MCT; catalyzes the formation of4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and2-C-methyl-D-erythritol 4-phosphate; involved inisoprenoid and isopentenyl-PP biosynthesis; formshomodimers
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-ispD
plasmid size
2722
insert size
699
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE