rpsE
30S ribosomal protein S5BBF10K_000797
source
Bacillus subtilis 168
located at the back of the 30S subunit body whereit stabilizes the conformation of the head with respect tothe body; contacts S4 and S8; with S4 and S12 plays a rolein translational accuracy; mutations in this gene resultin spectinomycin resistance
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-rpsE
plasmid size
2524
insert size
501
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE