rpoA

DNA-directed RNA polymerase subunit alpha
BBF10K_000805
source
Bacillus subtilis 168

catalyzes the transcription of DNA into RNA usingthe four ribonucleoside triphosphates as substrates.Dimerization of the alpha subunit is the first step in thesequential assembly of subunits to form the holoenzyme

attr.
Keoni Gandall

Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-rpoA
plasmid size
2968
insert size
945
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.