nadE
NH(3)-dependent NAD(+) synthetaseBBF10K_000812
source
Bacillus subtilis 168
Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-nadE
plasmid size
2842
insert size
819
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value![](https://freegenes.github.io/genes/images/BBF10K_000812.png)
References
Available Elsewhere
FALSE