ddl
D-alanine--D-alanine ligaseBBF10K_000814
source
Bacillus subtilis 168
D-alanine--D-alanine ligase; DdlA; DdlB;cytoplasmic; catalyzes the formation of D-alanyl-D-alaninefrom two D-alanines in peptidoglycan synthesis; there aretwo forms of this enzyme in Escherichia coli
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-ddl
plasmid size
3088
insert size
1065
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE