groES

chaperonin
BBF10K_000821
source
Bacillus subtilis 168

10 kDa chaperonin; Cpn10; GroES; formshomoheptameric ring; binds to one or both ends of theGroEL double barrel in the presence of adenine nucleotidescapping it; folding of unfolded substrates initiates in aGroEL-substrate bound and capped by GroES; release of thefolded substrate is dependent on ATP binding andhydrolysis in the trans ring

attr.
Keoni Gandall

Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-groES
plasmid size
2308
insert size
285
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.