groES
chaperoninBBF10K_000821
source
Bacillus subtilis 168
10 kDa chaperonin; Cpn10; GroES; formshomoheptameric ring; binds to one or both ends of theGroEL double barrel in the presence of adenine nucleotidescapping it; folding of unfolded substrates initiates in aGroEL-substrate bound and capped by GroES; release of thefolded substrate is dependent on ATP binding andhydrolysis in the trans ring
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-groES
plasmid size
2308
insert size
285
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE