murD
UDP-N-acetylmuramoylalanine--D-glutamate ligaseBBF10K_000861
source
Bacillus subtilis 168
UDP-N-acetylmuramoylalanine--D-glutamate ligase;involved in peptidoglycan biosynthesis; cytoplasmic;catalyzes the addition of glutamate to the nucleotideprecursor UDP-N-acetylmuramoyl-L-alanine during cell wallformation
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-murD
plasmid size
3379
insert size
1356
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE