rnc
ribonuclease 3BBF10K_000877
source
Bacillus subtilis 168
cytoplasmic enzyme involved in processing rRNA andsome mRNAs; substrates typically have dsRNA regions; formsa homodimer; have N-terminal nuclease and C-terminalRNA-binding domains; requires magnesium as preferred ionfor activity
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-rnc
plasmid size
2770
insert size
750
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE