frr
ribosome-recycling factorBBF10K_000887
source
Bacillus subtilis 168
Rrf; Frr; ribosome-recycling factor; release factor4; RF4; recycles ribosomes upon translation terminationalong with release factor RF-3 and elongation factor EF-G;A GTPase-dependent process results in release of 50S from70S; inhibited by release factor RF-1; essential forviability; structurally similar to tRNAs
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-frr
plasmid size
2578
insert size
558
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE