yneS
glycerol-3-phosphate acyltransferaseBBF10K_000906
source
Bacillus subtilis 168
involved in acylation of glycerol-3-phosphate toform 1-acyl-glycerol-3 phosphate for use in phospholipidbiosynthesis; functions with PlsX
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-yneS
plasmid size
2605
insert size
582
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No ValueReferences
Available Elsewhere
FALSE