engA

GTPase Der
BBF10K_000926
source
Bacillus subtilis 168

EngA; essential Neisserial GTPase; synchronizescellular events by interacting with multiple targets withtandem G-domains; overexpression in Escherichia colisuppresses rrmJ mutation; structural analysis of theThermotoga maritima ortholog shows different nucleotidebinding affinities in the two binding domains

attr.
Keoni Gandall

Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-engA
plasmid size
3334
insert size
1311
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.