rghRA
HTH-type transcriptional repressor RghRBBF10K_001004
source
Bacillus subtilis 168
Represses the expression of yvaM and both rapG and rapH. Binds directly to the promoter regions of yvaM, rapG and rapH.
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-rghRA
plasmid size
2428
insert size
408
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE