csrA
pleiotropic regulatory protein for carbon sourcemetabolismBBF10K_001092
source
Escherichia coli str. K-12 substr. MG1655
Binds to and is sequestered by non-coding small RNAs (sRNA) CsrB and CsrC which antagonize the activity of CsrA (PubMed:9211896, PubMed:12694612). The consensus RNA-binding site is CAGGA(U/A/C)G which is located in probable hairpin loops (PubMed:9211896). There are 18 sites in CsrB, which cooperatively binds about 18 copies of CsrA (PubMed:9211896, PubMed:12694612). CsrC has 9 sites, and cooperatively binds multiple copies of CsrA (PubMed:12694612). Indirectly activates expression of CsrB and CsrC, both dependently and independently of the BarA-UvrY two-component system (PubMed:12193630, PubMed:12694612). ppGpp activates transcription of CsrA-antagonistic small RNAs CsrB and CsrC, which downregulates CsrA's action on translation during the stringent response (PubMed:21488981).
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-csrA
plasmid size
2381
insert size
186
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE