ispD
4-diphosphocytidyl-2C-methyl-D-erythritolsynthaseBBF10K_001095
source
Escherichia coli str. K-12 substr. MG1655
Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-ispD
plasmid size
2834
insert size
711
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE