ftsB

cell division protein
BBF10K_001096

source

Escherichia coli str. K-12 substr. MG1655

Essential cell division protein. May link together the upstream cell division proteins, which are predominantly cytoplasmic, with the downstream cell division proteins, which are predominantly periplasmic.

attr.

Keoni Gandall

Usage

growth

shipping strain

{shipping_strain}

growth conditions

37 C, shaking 300 rpm

antibiotic

ampicillin

expression

strain

N/A

promoter

N/A

inducer

N/A

cloning

method

GoldenGate

enzyme

BsaI

overhangs

3' - AATG … GCTT - 5'

sequencing

forward primer

M13 For

reverse primer

M13 Rev

Construct

plasmid name

pOpen-ftsB

plasmid size

2435

insert size

312

origin

ColE1 High Copy

copy number

500-700

genbank file

Safety

BSL

BSL1

other information

No Value

References

primary references

NCBI

NP_417228.1

UniProt

Q46894

Available Elsewhere

FALSE

protocols and instructions

https://docs.google.com/document/d/1c8PyT1Z9cWYUif4XQzcWl8MPbqVuL8-RNKCAancaVQU/edit

citations

[1] Calles, J., Justice, I., Brinkley, D., Garcia, A. & Endy, D. Fail-safe genetic codes designed to intrinsically contain engineered organisms. Nucleic Acids Res. (2019). doi:10.1093/nar/gkz745 [2] Baba, T. et al. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2, 2006.0008 (2006)

intellectual property

We are unaware of third-party property rights claims on uses of this item.