yhhQ
DUF165 family inner membrane proteinBBF10K_001175
source
Escherichia coli str. K-12 substr. MG1655
Involved in the import of queuosine (Q) precursors, required for Q precursor salvage. Transports 7-cyano-7-deazaguanine (preQ(0)) and 7-aminomethyl-7-deazaguanine (preQ(1)), with a preference for preQ(0).
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-yhhQ
plasmid size
2789
insert size
666
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE