rplV
50S ribosomal protein L22BBF10K_001361
source
Mesoplasma florum L1
binds specifically to 23S rRNA during the earlystages of 50S assembly; makes contact with all 6 domainsof the 23S rRNA in the assembled 50S subunit and ribosome;mutations in this gene result in erythromycin resistance;located near peptidyl-transferase center
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-rplV
plasmid size
2402
insert size
339
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE