rpmJ
50S ribosomal protein L36BBF10K_001380
source
Mesoplasma florum L1
smallest protein in the large subunit; similar towhat is found with protein L31 and L33 several bacterialgenomes contain paralogs which may be regulated by zinc;the protein from Thermus thermophilus has a zinc-bindingmotif and contains a bound zinc ion; the proteins in thisgroup have the motif
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-rpmJ
plasmid size
2301
insert size
114
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE