ruvB
Holliday junction DNA helicase RuvBBBF10K_001655
source
Mesoplasma florum L1
promotes strand exchange during homologousrecombination; RuvAB complex promotes branch migration;RuvABC complex scans the DNA during branch migration andresolves Holliday junctions at consensus sequences; formshexameric rings around opposite DNA arms; requires ATP forbranch migration and orientation of RuvAB complexdetermines direction of migration
attr.
Keoni Gandall
Usage
growth
shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin
expression
strain
N/A
promoter
N/A
inducer
N/A
cloning
method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'
sequencing
forward primer
M13 For
reverse primer
M13 Rev
Construct
plasmid name
pOpen-ruvB
plasmid size
3011
insert size
948
origin
ColE1 High Copy
copy number
500-700
Safety
BSL
BSL1
other information
No Value
References
Available Elsewhere
FALSE