ruvB

Holliday junction DNA helicase RuvB
BBF10K_001655
source
Mesoplasma florum L1

promotes strand exchange during homologousrecombination; RuvAB complex promotes branch migration;RuvABC complex scans the DNA during branch migration andresolves Holliday junctions at consensus sequences; formshexameric rings around opposite DNA arms; requires ATP forbranch migration and orientation of RuvAB complexdetermines direction of migration

attr.
Keoni Gandall

Usage

growth

shipping strain
{shipping_strain}
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-ruvB
plasmid size
3011
insert size
948
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.